Day 10 - the Witch of the fume hood

Day 10 - the Witch of the fume hood

That day, Wang Li taught us how to make LB plate for growing bacteria. There are so many autoclaving (sterilization) steps involved to ensure that the plates are not contaminated by bacteria.

We started with weighing the agar and the LB powder, followed by addition of RO water and autoclave inside the machine. After that, we had to pour the LB plate solution to the petridish inside a fume hood that had been sterilized using UV light for 15 minutes to kill all the bacteria.

When we came to the fume hood, we saw that the UV light was already switched on, so we thought that someone may has forgotten to switch it off. Since the fume hood was shared by the whole lab, we just thought that we can use it and that's what we did. We just switched the UV light off and add Ampicillin to the solution and start pouring it to the petridishes.

Suddenly, a skinny middle-aged women appeared behind us and without any hesitation she just said in a nagging voice "Do you know that you can let the agar solidified outside the fumehood?" In other words, she was trying to get us out of the fumehood. In the end, we did not finish making the agar plate because of her.

I asked Ni whether that fume hood belong to a specific professor and Ni said it was shared for the whole lab. Wang Li told us that she was always there everyday before 5, that's why she thought that the fume hood belongs to her... What an irritating old egoistic witch she was... Sigh