Day 7 - Lab's inspection

Day 7 - Lab's inspection

In the morning, we continued with our plasmid isolation and proceed to restriction digest (RE digest). Basically what we do in RE digest is to digest a recombinant plasmid with some enzymes to confirm the presence of DNA insert in the plasmid. However, I forgot to label my tubes and Rei Juan took them out... Sigh.... So, my plasmids were not digested properly.... I re-did my RE digest in the end...

Ni also asked us to redo our PCR until we could get reproducible results. We had to be able to get the same result 3 times. We did PCR twice with the same samples today and both of them look different... Sigh....

During lunch, Ni suddenly became very excited and start talking about her past idols such as Britney Spears. She then started singing "Baby One More Time" which make Shen Qian and I frozen in terror. It was in the canteen during lunch hour... Many people will look at us and thinking that we were too stressed (which may be true)...

While we were autoclaving the pipette tips, a plump lady came around and started to talk in Chinese with Ming you. Shen Qian whispered to me that she was inspecting our lab. Based on Shen Qian translation, that plump woman were telling Mingyou that he was not supposed to put any personal belongings on his table such as his laptop (which was always there) and his place was too messy. Shen Qian also told me that she heard from someone that the inspector dislike Ni because she always wear skirt in lab (we are supposed to wear long pants and covered shoes in lab). Luckily Ni was not around as she went somewhere else and she was wearing a long flowing skirt today. But this time her skirt was longer and she wear a covered shoes instead of her usual pink look-like-Croc sandals.